Description: Rabbit polyclonal antibody to Cdc4 Immunogen: Recombinant full length protein of human Cdc4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FBXW7 Alternative Names: FBW7; FBX30; SEL10; F-box/WD repeat-containing protein 7; Archipelago homolog; hAgo; F-box and WD-40 domain-containing protein 7; F-box protein FBX30; SEL-10; hCdc4Entrez Gene (Human): 55294Entrez Gene (Mouse) : 50754SwissProt (Human): Q969H0SwissProt (Mouse) : Q8VBV4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cdc4 expression in mouse heart (A) whole cell lysates.
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Immunohistochemical analysis of Cdc4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.