Description: Rabbit polyclonal antibody to UCP1 Immunogen: Recombinant full length protein of human UCP1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: UCP1 Alternative Names: SLC25A7; UCP; Mitochondrial brown fat uncoupling protein 1; UCP 1; Solute carrier family 25 member 7; ThermogeninEntrez Gene (Human): 7350Entrez Gene (Mouse) : 22227Entrez Gene (Rat) : 24860SwissProt (Human): P25874SwissProt (Mouse) : P12242SwissProt (Rat) : P04633Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of UCP1 expression in HL60 (A), mouse kidney (B) whole cell lysates.
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Immunohistochemical analysis of UCP1 staining in rat fat formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of UCP1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.