Description: Rabbit polyclonal antibody to INSL3 Immunogen: Recombinant full length protein of human INSL3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: INSL3 Alternative Names: RLF; RLNL; Insulin-like 3; Leydig insulin-like peptide; Ley-I-L; Relaxin-like factorEntrez Gene (Human): 3640Entrez Gene (Rat) : 114215SwissProt (Human): P51460SwissProt (Mouse) : O09107SwissProt (Rat) : Q9WUK0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of INSL3 expression in U937 (A) whole cell lysates.
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  Immunohistochemical analysis of INSL3 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.