Description: Rabbit polyclonal antibody to ATPIF1 Immunogen: Recombinant full length protein of human ATPIF1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: ATPIF1 Alternative Names: ATPI; ATPase inhibitor mitochondrial; Inhibitor of F(1)F(o)-ATPase; IF(1); IF1Entrez Gene (Human): 93974Entrez Gene (Mouse) : 11983SwissProt (Human): Q9UII2SwissProt (Mouse) : O35143Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ATPIF1 expression in MCF7 (A), Hela (B), mouse brain (C) whole cell lysates.
-
Immunofluorescent analysis of ATPIF1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).