Description: Rabbit polyclonal antibody to COTL1 Immunogen: Recombinant full length protein of human COTL1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: COTL1 Alternative Names: CLP; Coactosin-like proteinEntrez Gene (Human): 23406Entrez Gene (Mouse) : 72042Entrez Gene (Rat) : 361422SwissProt (Human): Q14019SwissProt (Mouse) : Q9CQI6SwissProt (Rat) : B0BNA5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of COTL1 expression in Jurkat (A), Hela (B), mouse lung (C) whole cell lysates.
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Immunohistochemical analysis of COTL1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.