Description: Rabbit polyclonal antibody to PHB2 Immunogen: Recombinant full length protein of human PHB2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: PHB2 Alternative Names: BAP; REA; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activityEntrez Gene (Human): 11331Entrez Gene (Mouse) : 12034Entrez Gene (Rat) : 114766SwissProt (Human): Q99623SwissProt (Mouse) : O35129SwissProt (Rat) : Q5XIH7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PHB2 expression in HepG2 (A), A549 (B), mouse hear (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of PHB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.