Description: Rabbit polyclonal antibody to NUDT21 Immunogen: Recombinant full length protein of human NUDT21 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NUDT21 Alternative Names: CFIM25; CPSF25; CPSF5; Cleavage and polyadenylation specificity factor subunit 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; CFIm25; CPSF 25 kDa subunit; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Pre-mRNA cleavage factor Im 25 kDa subunitEntrez Gene (Human): 11051Entrez Gene (Mouse) : 68219SwissProt (Human): O43809SwissProt (Mouse) : Q9CQF3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NUDT21 expression in SHSY5Y (A), HepG2 (B), mouse thymus (C) whole cell lysates.
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Immunohistochemical analysis of NUDT21 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.