Description: Rabbit polyclonal antibody to GALC Immunogen: Recombinant full length protein of human GALC Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GALC Alternative Names: Galactocerebrosidase; GALCERase; Galactocerebroside beta-galactosidase; Galactosylceramidase; Galactosylceramide beta-galactosidaseEntrez Gene (Human): 2581Entrez Gene (Mouse) : 14420SwissProt (Human): P54803SwissProt (Mouse) : P54818Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GALC expression in HepG2 (A), mouse brain (B), rat testis (C) whole cell lysates.
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Immunohistochemical analysis of GALC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.