Description: Rabbit polyclonal antibody to CYLD Immunogen: Recombinant full length protein of human CYLD Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CYLD Alternative Names: CYLD1; KIAA0849; Ubiquitin carboxyl-terminal hydrolase CYLD; Deubiquitinating enzyme CYLD; Ubiquitin thioesterase CYLD; Ubiquitin-specific-processing protease CYLDEntrez Gene (Human): 1540Entrez Gene (Mouse) : 74256Entrez Gene (Rat) : 312937SwissProt (Human): Q9NQC7SwissProt (Mouse) : Q80TQ2SwissProt (Rat) : Q66H62Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CYLD expression in Hela (A), mouse kidney (B) whole cell lysates.
-
Immunohistochemical analysis of CYLD staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.