Description: Rabbit polyclonal antibody to ATP6V1E1 Immunogen: Recombinant full length protein of human ATP6V1E1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50) Gene Symbol: ATP6V1E1 Alternative Names: ATP6E; ATP6E2; V-type proton ATPase subunit E 1; V-ATPase subunit E 1; V-ATPase 31 kDa subunit; p31; Vacuolar proton pump subunit E 1Entrez Gene (Human): 529Entrez Gene (Mouse) : 11973Entrez Gene (Rat) : 297566SwissProt (Human): P36543SwissProt (Mouse) : P50518SwissProt (Rat) : Q6PCU2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATP6V1E1 expression in LO2 (A), mouse liver (B), rat brain (C) whole cell lysates.
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Immunofluorescent analysis of ATP6V1E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).