Description: Rabbit polyclonal antibody to Sam50 Immunogen: Recombinant full length protein of human Sam50 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SAMM50 Alternative Names: SAM50; Sorting and assembly machinery component 50 homolog; Transformation-related gene 3 protein; TRG-3Entrez Gene (Human): 25813Entrez Gene (Mouse) : 68653Entrez Gene (Rat) : 300111SwissProt (Human): Q9Y512SwissProt (Mouse) : Q8BGH2SwissProt (Rat) : Q6AXV4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Sam50 expression in A431 (A), HL60 (B), mouse heart (C) whole cell lysates.
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Immunohistochemical analysis of Sam50 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.