Description: Rabbit polyclonal antibody to KIF5A Immunogen: Recombinant full length protein of human KIF5A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: KIF5A Alternative Names: NKHC1; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; NKHCEntrez Gene (Human): 3798Entrez Gene (Mouse) : 16572Entrez Gene (Rat) : 314906SwissProt (Human): Q12840SwissProt (Mouse) : P33175SwissProt (Rat) : Q6QLM7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of KIF5A expression in mouse brain (A), rat brain (B) whole cell lysates.
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  Immunohistochemical analysis of KIF5A staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of KIF5A staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).