Description: Rabbit polyclonal antibody to UBC Immunogen: KLH-conjugated synthetic peptide of human UBC Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: UBC Alternative Names: Polyubiquitin-CEntrez Gene (Human): 7316Entrez Gene (Mouse) : 22190Entrez Gene (Rat) : 50522SwissProt (Human): P0CG48SwissProt (Mouse) : P0CG50SwissProt (Rat) : Q63429Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of UBC expression in HepG2 (A), Hela (B) whole cell lysates.
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Immunohistochemical analysis of UBC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.