Description: Rabbit polyclonal antibody to Neurotrophin 3 Immunogen: Recombinant full length protein of human Neurotrophin 3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NTF3 Alternative Names: Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factorEntrez Gene (Human): 4908Entrez Gene (Mouse) : 18205SwissProt (Human): P20783SwissProt (Mouse) : P20181Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Neurotrophin 3 expression in HT29 (A) whole cell lysates.
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Immunohistochemical analysis of Neurotrophin 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.