Description: Rabbit polyclonal antibody to Histone H3 (MonoMethyl-R26) Immunogen: KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R26 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50), ChIP (1/10 - 1/50) Gene Symbol: HIST3H3 Alternative Names: H3FT; Histone H3.1t; H3/t; H3t; H3/gEntrez Gene (Human): 8290SwissProt (Human): Q16695Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone H3 (MonoMethyl-R26) expression in Hela (A) whole cell lysates.
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Immunofluorescent analysis of Histone H3 (MonoMethyl-R26) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.