Description: Rabbit polyclonal antibody to Neurofascin Immunogen: Recombinant full length protein of human Neurofascin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NFASC Alternative Names: KIAA0756; NeurofascinEntrez Gene (Human): 23114Entrez Gene (Mouse) : 269116Entrez Gene (Rat) : 116690SwissProt (Human): O94856SwissProt (Mouse) : Q810U3SwissProt (Rat) : P97685Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Neurofascin expression in Hela (A), HL60 (B), mouse brain (C), rat brain (D) whole cell lysates.
-
Immunohistochemical analysis of Neurofascin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.