Description: Rabbit polyclonal antibody to TCP1 alpha Immunogen: Recombinant full length protein of human TCP1 alpha Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TCP1 Alternative Names: CCT1; CCTA; T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alphaEntrez Gene (Human): 6950Entrez Gene (Mouse) : 21454Entrez Gene (Rat) : 24818SwissProt (Human): P17987SwissProt (Mouse) : P11983SwissProt (Rat) : P28480Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TCP1 alpha expression in PC3 (A), Hela (B), Jurkat (C) whole cell lysates.
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Immunohistochemical analysis of TCP1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.