Description: Rabbit polyclonal antibody to TMPase Immunogen: Recombinant full length protein of human TMPase Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ACPP Alternative Names: Prostatic acid phosphatase; PAP; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPaseEntrez Gene (Human): 55Entrez Gene (Mouse) : 56318Entrez Gene (Rat) : 56780SwissProt (Human): P15309SwissProt (Mouse) : Q8CE08SwissProt (Rat) : P20646Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TMPase expression in MCF7 (A) whole cell lysates.
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Immunohistochemical analysis of TMPase staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.