Description: Rabbit polyclonal antibody to HBEGF Immunogen: Recombinant full length protein of human HBEGF Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HBEGF Alternative Names: DTR; DTS; HEGFL; Proheparin-binding EGF-like growth factorEntrez Gene (Human): 1839Entrez Gene (Mouse) : 15200Entrez Gene (Rat) : 25433SwissProt (Human): Q99075SwissProt (Mouse) : Q06186SwissProt (Rat) : Q06175Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HBEGF expression in U87 (A), mouse skeletal muscle (B) whole cell lysates.
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  Immunohistochemical analysis of HBEGF staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.