Description: Rabbit polyclonal antibody to Ghrelin Immunogen: Recombinant full length protein of human Ghrelin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GHRL Alternative Names: MTLRP; Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Entrez Gene (Human): 51738Entrez Gene (Rat) : 59301SwissProt (Human): Q9UBU3SwissProt (Rat) : Q9QYH7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ghrelin expression in Hela (A) whole cell lysates.
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Immunohistochemical analysis of Ghrelin staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.