Description: Rabbit polyclonal antibody to Apolipoprotein B Immunogen: Recombinant full length protein of human Apolipoprotein B Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: APOB Alternative Names: Apolipoprotein B-100; Apo B-100Entrez Gene (Human): 338Entrez Gene (Mouse) : 238055Entrez Gene (Rat) : 54225SwissProt (Human): P04114SwissProt (Mouse) : E9Q414SwissProt (Rat) : Q7TMA5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Apolipoprotein B expression in HepG2 (A), HL60 (B), mouse liver (C), rat kidney (D) whole cell lysates.
-
Immunofluorescent analysis of Apolipoprotein B staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures