Description: Rabbit polyclonal antibody to COX2 Immunogen: KLH-conjugated synthetic peptide of human COX2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MT-CO2 Alternative Names: COII; COXII; MTCO2; Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide IIEntrez Gene (Human): 4513Entrez Gene (Mouse) : 17709SwissProt (Human): P00403SwissProt (Mouse) : P00405SwissProt (Rat) : P00406Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of COX2 expression in mouse liver (A), mouse heart (B), rat liver (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of COX2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.