Description: Rabbit polyclonal antibody to LC3-I/II Immunogen: Recombinant full length protein of human LC3-I/II Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MAP1LC3A Alternative Names: Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alphaEntrez Gene (Human): 84557Entrez Gene (Mouse) : 66734Entrez Gene (Rat) : 362245SwissProt (Human): Q9H492SwissProt (Mouse) : Q91VR7SwissProt (Rat) : Q6XVN8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of LC3-I/II expression in mouse brain (A), rat brain (B) whole cell lysates.
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  Immunohistochemical analysis of LC3-I/II staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.