Anti-HO-1 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to HO-1 Immunogen: Recombinant full length protein of human HO-1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: HMOX1 Alternative Names: HO; HO1; Heme oxygenase 1; HO-1Entrez Gene (Human): 3162Entrez Gene (Mouse) : 15368Entrez Gene (Rat) : 24451SwissProt (Human): P09601SwissProt (Mouse) : P14901SwissProt (Rat) : P06762Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HO-1 expression in Hela (A), mouse spleen (B), rat spleen (C) whole cell lysates. -
Immunofluorescent analysis of HO-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PM2. 5 leads to adverse pregnancy outcomes by inducing trophoblast oxidative stress and mitochondrial apoptosis via KLF9/CYP1A1 transcriptional axis