Description: Rabbit polyclonal antibody to GPR49 Immunogen: Recombinant full length protein of human GPR49 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: LGR5 Alternative Names: GPR49; GPR67; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38Entrez Gene (Human): 8549Entrez Gene (Mouse) : 14160Entrez Gene (Rat) : 299802SwissProt (Human): O75473SwissProt (Mouse) : Q9Z1P4SwissProt (Rat) : D4AC13Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GPR49 expression in A431 (A), mouse brain (B), rat skeletal muscle (C) whole cell lysates.
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  Immunohistochemical analysis of GPR49 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.