Description: Rabbit polyclonal antibody to Semaphorin 4F Immunogen: Recombinant full length protein of human Semaphorin 4F Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SEMA4F Alternative Names: SEMAM; SEMAW; Semaphorin-4F; Semaphorin-M; Sema M; Semaphorin-W; Sema WEntrez Gene (Human): 10505Entrez Gene (Mouse) : 20355Entrez Gene (Rat) : 29745SwissProt (Human): O95754SwissProt (Mouse) : Q9Z123SwissProt (Rat) : Q9Z143Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Semaphorin 4F expression in HepG2 (A), A549 (B), mouse brain (C), mouse lung (D), rat liver (E) whole cell lysates.
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  Immunohistochemical analysis of Semaphorin 4F staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.