Description: Rabbit polyclonal antibody to NCOA2 Immunogen: Recombinant full length protein of human NCOA2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NCOA2 Alternative Names: BHLHE75; SRC2; TIF2; Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2Entrez Gene (Human): 10499Entrez Gene (Mouse) : 17978Entrez Gene (Rat) : 83724SwissProt (Human): Q15596SwissProt (Mouse) : Q61026SwissProt (Rat) : Q9WUI9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NCOA2 expression in Hela (A), A549 (B), BT474 (C) whole cell lysates.
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Immunohistochemical analysis of NCOA2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.