Description: Rabbit polyclonal antibody to EIF3B Immunogen: Recombinant full length protein of human EIF3B Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: EIF3B Alternative Names: EIF3S9; Eukaryotic translation initiation factor 3 subunit B; eIF3b; Eukaryotic translation initiation factor 3 subunit 9; Prt1 homolog; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116Entrez Gene (Human): 8662Entrez Gene (Mouse) : 27979Entrez Gene (Rat) : 288516SwissProt (Human): P55884SwissProt (Mouse) : Q8JZQ9SwissProt (Rat) : Q4G061Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF3B expression in Hela (A), Jurkat (B), NIH3T3 (C), rat thymus (D) whole cell lysates.
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Immunofluorescent analysis of EIF3B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).