Description: Rabbit polyclonal antibody to SLC11A2 Immunogen: Recombinant full length protein of human SLC11A2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: SLC11A2 Alternative Names: DCT1; DMT1; NRAMP2; Natural resistance-associated macrophage protein 2; NRAMP 2; Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; Solute carrier family 11 member 2Entrez Gene (Human): 4891Entrez Gene (Mouse) : 18174Entrez Gene (Rat) : 25715SwissProt (Human): P49281SwissProt (Mouse) : P49282SwissProt (Rat) : O54902Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SLC11A2 expression in SW620 (A), mouse kidney (B), mouse spleen (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of SLC11A2 staining in rat stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of SLC11A2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).