Description: Rabbit polyclonal antibody to HINT1 Immunogen: Recombinant full length protein of human HINT1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: HINT1 Alternative Names: HINT; PKCI1; PRKCNH1; Histidine triad nucleotide-binding protein 1; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1Entrez Gene (Human): 3094Entrez Gene (Mouse) : 15254Entrez Gene (Rat) : 690660SwissProt (Human): P49773SwissProt (Mouse) : P70349SwissProt (Rat) : P62959Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HINT1 expression in Hela (A), K562 (B), NIH3T3 (C), mouse kidney (D), rat liver (E) whole cell lysates.
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Immunofluorescent analysis of HINT1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).