Description: Rabbit polyclonal antibody to IKAP Immunogen: Recombinant full length protein of human IKAP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: IKBKAP Alternative Names: ELP1; IKAP; Elongator complex protein 1; ELP1; IkappaB kinase complex-associated protein; IKK complex-associated protein; p150Entrez Gene (Human): 8518Entrez Gene (Mouse) : 230233Entrez Gene (Rat) : 140934SwissProt (Human): O95163SwissProt (Mouse) : Q7TT37SwissProt (Rat) : Q8VHU4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of IKAP expression in Hela (A), HepG2 (B), mouse liver (C), rat liver (D) whole cell lysates.
-
Immunohistochemical analysis of IKAP staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.