Description: Rabbit polyclonal antibody to RAP1A Immunogen: KLH-conjugated synthetic peptide of human RAP1A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RAP1A Alternative Names: KREV1; Ras-related protein Rap-1A; C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1Entrez Gene (Human): 5906Entrez Gene (Mouse) : 109905Entrez Gene (Rat) : 295347SwissProt (Human): P62834SwissProt (Mouse) : P62835SwissProt (Rat) : P62836Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RAP1A expression in HL60 (A), mouse lung (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of RAP1A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.