Description: Rabbit polyclonal antibody to PPP3R1 Immunogen: Recombinant full length protein of human PPP3R1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PPP3R1 Alternative Names: CNA2; CNB; Calcineurin subunit B type 1; Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1Entrez Gene (Human): 5534Entrez Gene (Mouse) : 19058Entrez Gene (Rat) : 29748SwissProt (Human): P63098SwissProt (Mouse) : Q63810SwissProt (Rat) : P63100Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPP3R1 expression in SW480 (A), MCF7 (B), mouse brain (C), rat testis (D) whole cell lysates.
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Immunofluorescent analysis of PPP3R1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).