Description: Rabbit polyclonal antibody to N-myc Immunogen: Recombinant full length protein of human N-myc Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MYCN Alternative Names: BHLHE37; NMYC; N-myc proto-oncogene protein; Class E basic helix-loop-helix protein 37; bHLHe37Entrez Gene (Human): 4613Entrez Gene (Mouse) : 18109Entrez Gene (Rat) : 298894SwissProt (Human): P04198SwissProt (Mouse) : P03966SwissProt (Rat) : Q63379Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of N-myc expression in mouse brain (A) whole cell lysates.
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Immunohistochemical analysis of N-myc staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.