Description: Rabbit polyclonal antibody to U2AF1 Immunogen: Recombinant full length protein of human U2AF1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: U2AF1 Alternative Names: U2AF35; U2AFBP; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunitEntrez Gene (Human): 102724594; 7307Entrez Gene (Mouse) : 108121SwissProt (Human): Q01081SwissProt (Mouse) : Q9D883Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of U2AF1 expression in A549 (A), MCF7 (B), mouse pancreas (C), rat liver (D) whole cell lysates.
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  Immunohistochemical analysis of U2AF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.