Description: Rabbit polyclonal antibody to Transketolase Immunogen: Recombinant full length protein of human Transketolase Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TKT Alternative Names: Transketolase; TKEntrez Gene (Human): 7086Entrez Gene (Mouse) : 21881Entrez Gene (Rat) : 64524SwissProt (Human): P29401SwissProt (Mouse) : P40142SwissProt (Rat) : P50137Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Transketolase expression in Jurkat (A), MCF7 (B), mouse liver (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of Transketolase staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.