Description: Rabbit polyclonal antibody to TIA1 Immunogen: Recombinant full length protein of human TIA1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TIA1 Alternative Names: Nucleolysin TIA-1 isoform p40; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1Entrez Gene (Human): 7072Entrez Gene (Mouse) : 21841SwissProt (Human): P31483SwissProt (Mouse) : P52912Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TIA1 expression in SKOV3 (A), Hela (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of TIA1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.