Description: Rabbit polyclonal antibody to SUMO4 Immunogen: KLH-conjugated synthetic peptide of human SUMO4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SUMO4 Alternative Names: SMT3H4; Small ubiquitin-related modifier 4; SUMO-4; Small ubiquitin-like protein 4Entrez Gene (Human): 387082SwissProt (Human): Q6EEV6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SUMO4 expression in Jurkat (A), MCF7 (B) whole cell lysates.
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Immunohistochemical analysis of SUMO4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.