Description: Rabbit polyclonal antibody to STX Immunogen: Recombinant full length protein of human STX Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/100) Gene Symbol: ST8SIA2 Alternative Names: SIAT8B; STX; Alpha-2,8-sialyltransferase 8B; Sialyltransferase 8B; SIAT8-B; Sialyltransferase St8Sia II; ST8SiaII; Sialyltransferase X; STXEntrez Gene (Human): 8128Entrez Gene (Mouse) : 20450Entrez Gene (Rat) : 117523SwissProt (Human): Q92186SwissProt (Mouse) : O35696SwissProt (Rat) : Q07977Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STX expression in THP1 (A), SKOV3 (B), mouse brain (C), mouse lung (D), rat heart (E) whole cell lysates.
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Immunohistochemical analysis of STX staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.