Description: Rabbit polyclonal antibody to SMARCAD1 Immunogen: Recombinant full length protein of human SMARCAD1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: SMARCAD1 Alternative Names: KIAA1122; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1Entrez Gene (Human): 56916Entrez Gene (Mouse) : 13990Entrez Gene (Rat) : 312398SwissProt (Human): Q9H4L7SwissProt (Mouse) : Q04692SwissProt (Rat) : D3Z9Z9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SMARCAD1 expression in A549 (A), HepG2 (B), mouse kidney (C), mouse lung (D) whole cell lysates.
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Immunofluorescent analysis of SMARCAD1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.