Description: Rabbit polyclonal antibody to SIRT4 Immunogen: KLH-conjugated synthetic peptide of human SIRT4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SIRT4 Alternative Names: SIR2L4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent ADP-ribosyltransferase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4Entrez Gene (Human): 23409Entrez Gene (Mouse) : 75387SwissProt (Human): Q9Y6E7SwissProt (Mouse) : Q8R216Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of SIRT4 expression in A549 (A), NIH3T3 (B), mouse testis (C) whole cell lysates.
-
Immunohistochemical analysis of SIRT4 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.