Description: Rabbit polyclonal antibody to RUFY1 Immunogen: Recombinant full length protein of human RUFY1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/100) Gene Symbol: RUFY1 Alternative Names: RABIP4; ZFYVE12; RUN and FYVE domain-containing protein 1; FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12Entrez Gene (Human): 80230Entrez Gene (Mouse) : 216724SwissProt (Human): Q96T51SwissProt (Mouse) : Q8BIJ7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RUFY1 expression in mouse kidney (A), mouse heart (B), rat lung (C) whole cell lysates.
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  Immunohistochemical analysis of RUFY1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.