Description: Rabbit polyclonal antibody to RPS10 Immunogen: Recombinant full length protein of human RPS10 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/50), IP (1/20 - 1/50) Gene Symbol: RPS10 Alternative Names: 40S ribosomal protein S10Entrez Gene (Human): 6204Entrez Gene (Mouse) : 67097Entrez Gene (Rat) : 100363439; 81773SwissProt (Human): P46783SwissProt (Mouse) : P63325SwissProt (Rat) : P63326Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RPS10 expression in A549 (A), HT29 (B), mouse spleen (C), mouse heart (D) whole cell lysates.
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  Immunohistochemical analysis of RPS10 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of RPS10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.