Description: Rabbit polyclonal antibody to RNF5 Immunogen: Recombinant full length protein of human RNF5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RNF5 Alternative Names: G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog; HsRma1Entrez Gene (Human): 6048Entrez Gene (Mouse) : 54197Entrez Gene (Rat) : 407784SwissProt (Human): Q99942SwissProt (Mouse) : O35445SwissProt (Rat) : Q5M807Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RNF5 expression in MCF7 (A), Jurkat (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates.
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Immunohistochemical analysis of RNF5 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.