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Anti-RNF5 Antibody CQA2207
  • Western blot analysis of RNF5 expression in MCF7 (A), Jurkat (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates.
  • Immunohistochemical analysis of RNF5 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-RNF5 Antibody
Cat No: CQA2207
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
50 μl
$150
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Description: Rabbit polyclonal antibody to RNF5
Immunogen: Recombinant full length protein of human RNF5
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol: RNF5
Alternative Names: G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog; HsRma1
Entrez Gene (Human): 6048
Entrez Gene (Mouse) : 54197
Entrez Gene (Rat) : 407784
SwissProt (Human): Q99942
SwissProt (Mouse) : O35445
SwissProt (Rat) : Q5M807
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of RNF5 expression in MCF7 (A), Jurkat (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates.
  • Immunohistochemical analysis of RNF5 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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