Description: Rabbit polyclonal antibody to RBPJ Immunogen: Recombinant full length protein of human RBPJ Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RBPJ Alternative Names: IGKJRB; IGKJRB1; RBPJK; RBPSUH; Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30Entrez Gene (Human): 3516Entrez Gene (Mouse) : 19664SwissProt (Human): Q06330SwissProt (Mouse) : P31266Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RBPJ expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of RBPJ staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.