Description: Rabbit polyclonal antibody to PSMD13 Immunogen: Recombinant full length protein of human PSMD13 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PSMD13 Alternative Names: 26S proteasome non-ATPase regulatory subunit 13; 26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome regulatory subunit p40.5Entrez Gene (Human): 5719Entrez Gene (Mouse) : 23997Entrez Gene (Rat) : 365388SwissProt (Human): Q9UNM6SwissProt (Mouse) : Q9WVJ2SwissProt (Rat) : B0BN93Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMD13 expression in HepG2 (A), Jurkat (B), SW620 (C), mouse testis (D), rat lung (E) whole cell lysates.
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Immunofluorescent analysis of PSMD13 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.