Description: Rabbit polyclonal antibody to PPP2R1A Immunogen: Recombinant full length protein of human PPP2R1A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: PPP2R1A Alternative Names: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alphaEntrez Gene (Human): 5518Entrez Gene (Mouse) : 51792SwissProt (Human): P30153SwissProt (Mouse) : Q76MZ3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of PPP2R1A expression in SKOV3 (A), HepG2 (B), mouse brain (C), mouse kidney (D) whole cell lysates.
• 
  Immunofluorescent analysis of PPP2R1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).