Description: Rabbit polyclonal antibody to PLGF Immunogen: Recombinant full length protein of human PLGF Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PGF Alternative Names: PGFL; PLGF; Placenta growth factor; PlGFEntrez Gene (Human): 5228Entrez Gene (Mouse) : 18654Entrez Gene (Rat) : 94203SwissProt (Human): P49763SwissProt (Mouse) : P49764SwissProt (Rat) : Q63434Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLGF expression in BT474 (A), mouse brain (B) whole cell lysates.
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Immunohistochemical analysis of PLGF staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.