Description: Rabbit polyclonal antibody to Nucleolin Immunogen: Recombinant full length protein of human Nucleolin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NCL Alternative Names: Nucleolin; Protein C23Entrez Gene (Human): 4691Entrez Gene (Mouse) : 17975SwissProt (Human): P19338SwissProt (Mouse) : P09405Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Nucleolin expression in MCF7 (A), A549 (B), THP1 (C) whole cell lysates.
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Immunohistochemical analysis of Nucleolin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.