Description: Rabbit polyclonal antibody to NCK2 Immunogen: Recombinant full length protein of human NCK2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/100) Gene Symbol: NCK2 Alternative Names: GRB4; Cytoplasmic protein NCK2; Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-betaEntrez Gene (Human): 8440Entrez Gene (Mouse) : 17974SwissProt (Human): O43639SwissProt (Mouse) : O55033Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NCK2 expression in HEK293T (A), mouse lung (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of NCK2 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.